Composite

Part:BBa_K2082231:Design

Designed by: Pascal Schmidt   Group: iGEM16_Bielefeld-CeBiTec   (2016-10-08)


RFP under the control of an optimized lacZ promoter combined with the protein SH2:cI434


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 841
    Illegal NheI site found at 864
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 656
    Illegal AgeI site found at 768
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Badran et al. (2016) and Wojcik et al. (2010) were the main inspiration sources for creating this part.


Source

Gene synthesis of IDT for the creation of the reporter and the SH2:cI434 protein.

References

  • Badran, A. H., Guzov, V. M. & Huai, Q. et al. (2016) Continuous evolution of Bacillus thuringiensis toxins overcomes insect resistance. Nature 533 (7601), 58–63.
  • Wojcik, J., Hantschel, O. & Grebien, F. et al. (2010) A potent and highly specific FN3 monobody inhibitor of the Abl SH2 domain. Nature structural & molecular biology 17 (4), 519–527.